![]() ![]() ![]() Global market analysis: world agricultural production. USDA, 2020 UNITED STATES DEPARTMENT OF AGRICULTURE – USDA. The status of the paddy and rice industry in Malaysia. Malaysia constitutes only 0.37% of the total world rice production, which covers 72% of the national self-sufficiency level ( KR Institute, 2019 KHAZANAH RESEARCH INSTITUTE – KR Institute. Regrettably, the global trend of rice production in 2020 will represent a decrease trend compare to previous production year ( USDA, 2020 UNITED STATES DEPARTMENT OF AGRICULTURE – USDA. Identification of BLB resistant genes in some rice varieties for development of high yielding bacterial leaf blight tolerant types. About 3.5 billion world population are relying on rice as their main source of energy ( Majumder et al., 2019 MAJUMDER, K., MONDAL, S.J., MALLICK, R. Research Journal of Applied Sciences, Engineering and Technology, vol. Issues and challenges facing rice production and food security in the granary areas in the east coast economic region (ECER), Malaysia. Rice ( Oryza sativa L.) is always known to be at the frontline to tackle world hunger, as it is the second most widely grown cereal crop in the world ( Siwar et al., 2014 SIWAR, C., IDRIS, N.D., YASAR, M. retrocruzamento seleção assistida por marcadores gene Xa7 mancha bacteriana das folhas Este estudo demonstra uma história de sucesso de aplicação de MAS no desenvolvimento de linhas de arroz melhoradas com resistência a doenças contra a doença BLB. Duas linhas, PB-2-107 e PB-2-34, foram escolhidas como linhas potenciais por causa de seus desempenhos excelentes em comparação com a mãe, MR219. As linhas melhoradas exibiram características morfológicas e de desempenho de rendimento semelhantes às da variedade elite, MR219. O pai recorrente MR219 e a variedade controle, MR263, estavam gravemente infectados pela doença. No BC3F4, conseguimos gerar 19 linhagens introgressadas com o gene Xa7 homozigoto e apresentamos características de resistência como genitor doador quando desafiado com Xanthomonas oryzae pv. O gene de resistência a BLB de amplo espectro, Xa7 do pai doador IRBB7, foi introgressado no MR219 suscetível (pai recorrente) usando dois marcadores flanqueadores ID7 e ID15. Portanto, o objetivo deste estudo foi aumentar os caracteres BLB resistentes a doenças da variedade comercial MR219 de alto rendimento por meio de uma abordagem de cruzamento retrocruzamento com ferramenta de apoio de seleção assistida por marcador (MAS). O desenvolvimento de variedades de arroz que herdaram características de resistência ao BLB é uma abordagem crucial para promover e sustentar a indústria do arroz na Malásia. Essa doença causa perdas substanciais de rendimento de até 70%. backcrossing marker assisted selection Xa7 gene bacterial leaf blightĪ mancha bacteriana das folhas (BLB) é uma das principais doenças do arroz na Malásia. This study demonstrates a success story of MAS application in development of improved disease resistance lines of rice against BLB disease. Two lines, PB-2-107 and PB-2-34 were chosen to be potential lines because of their outstanding performances compared to parent, MR219. The improved lines exhibited similar morphological and yield performance characters as to the elite variety, MR219. Recurrent parent MR219 and control variety, MR263 were found to be severely infected by the disease. At BC 3F 4, we managed to generate 19 introgressed lines with homozygous Xa7 gene and showed resistant characteristics as donor parent when it was challenged with Xanthomonas oryzae pv. Broad spectrum BLB resistance gene, Xa7 from donor parent IRBB7 were introgressed into the susceptible MR219 (recurrent parent) using two flanking markers ID7 and ID15. ![]() Hence, this study aims were to enhance BLB disease resistant characters of high yielding commercial variety MR219 through backcross breeding approach with supporting tool of marker-assisted selection (MAS). Development of rice varieties which inherited BLB resistant traits is a crucial approach to promote and sustain rice industry in Malaysia. This disease causes substantial yield loss as high as 70%. Bacterial leaf blight (BLB) is one of the major rice diseases in Malaysia. ![]()
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